Molecular Biology and Pooling Design
نویسندگان
چکیده
The study of gene functions requires high quality of a DNA library. A large amount of testing and screening needs to be performed to obtain a highquality DNA library. Therefore, the efficiency of testing and screening becomes very important. Pooling design is a very helpful tool, which has developed a lot of applications in molecular biology. In this chapter, we introduce recent developments in this research direction.
منابع مشابه
Improved construction for pooling design
Pooling design is a mathematical tool with many applications in molecular biology, specially to reduce the number of tests for DNA library screening. In this note, we study construction of pooling design and present an improvement to a recent new construction given by Du et al. (J. Comput. Biol. 13:990–995, 2006).
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The study of gene functions requires a DNA library of high quality, such a library is obtained from a large mount of testing and screening. Pooling design is a very helpful tool for reducing the number of tests for DNA library screening. In this paper, we present new one- and two-stage pooling designs, together with new probabilistic pooling designs. The approach in this paper works for both er...
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The study of gene functions requires the development of a DNA library of high quality through much of testing and screening. Pooling design is a mathematical tool to reduce the number of tests for DNA library screening. The transversal design is a special type of pooling design, which is good in implementation. In this paper, we present a new construction for transversal designs. We will also e...
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Pooling designs are used in clone library screening to efficiently distinguish positive clones from negative clones. Mathematically, a pooling design is just a nonadaptive group testing scheme which has been extensively studied in the literature. In some applications, there is a third category of clones called "inhibitors" whose effect is to neutralize positives. Specifically, the presence of a...
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